Tritiated starch granules

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Tritiated starch granules.

1. Surface-labelling of starch granules by tritiation seems feasible, and a technique is described that could be useful in structure determination. The impurities that are produced must be taken into account but the fact that a high polymer can be successfully tritiated seems very promising. 2. The surfaces of corn-starch granules must contain both amylose and amylopectin.

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Micromanipulative Studies on Gelatinized Starch Granules.

Gelatinized starch granules are generally considered to be comprised of sacs or outer membranes of starchy material enclosing a more or less fluid colloidal starch dispersion [see reviews by RADLEY (10)] and KERR (6). NXGEI, (9) and MEYER (8) considered the membrane to be precxistent in the native, ungelatinized granule. ALSBERG (1) and BADENHUIZEN (2, 3) on the other hand, concluded that the m...

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Glucan affinity of starch synthase IIa determines binding of starch synthase I and starch-branching enzyme IIb to starch granules.

The sugary-2 mutation in maize (Zea mays L.) is a result of the loss of catalytic activity of the endosperm-specific SS (starch synthase) IIa isoform causing major alterations to amylopectin architecture. The present study reports a biochemical and molecular analysis of an allelic variant of the sugary-2 mutation expressing a catalytically inactive form of SSIIa and sheds new light on its centr...

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Recreating the synthesis of starch granules in yeast

Starch, as the major nutritional component of our staple crops and a feedstock for industry, is a vital plant product. It is composed of glucose polymers that form massive semi-crystalline granules. Its precise structure and composition determine its functionality and thus applications; however, there is no versatile model system allowing the relationships between the biosynthetic apparatus, gl...

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Starch-branching enzymes preferentially associated with A-type starch granules in wheat endosperm.

Two starch granule-bound proteins (SGP), SGP-140 and SGP-145, were preferentially associated with A-type starch granules (>10 microm) in developing and mature wheat (Triticum aestivum) kernels. Immunoblotting and N-terminal sequencing suggested that the two proteins were different variants of SBEIc, a 152-kD isoform of wheat starch-branching enzyme. Both SGP-140 and SGP-145 were localized to th...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1965

ISSN: 0006-2936

DOI: 10.1042/bj0960336